TABLE 2.

Position, name, function, PCR primers, and length sequenced for the 10 X. fastidiosa genes

Gene positiona	Gene IDa	Gene namea	Biochemical functiona	Primer sequence (forward, reverse)	KA/KSb	Gene length (bp)
XF0136	holC	DNA polymerase III holoenzyme chi subunit	Replication	5′-GATTTCCAAACCGCGCTTTC-3′, 5′-TCATGTGCAGGCCGCGTCTCT-3′	0.08	342
XF0257	rfbD	dTDP-4-dehydrorhamnose 3,5-epimerase	Surface polysaccharides	5′-TTTGGTGATTGAGCCGAGGGT-3′, 5′-CCATAAACGGCCGCTTTC-3′	0.29	429
XF0316	nuoL	NADH-ubiquinone oxidoreductase, NQO12 subunit	Aerobic respiration	5′-CATTATTGCCGGATTGTTAGG-3′, 5′-GCGGGAAACATTACCAAGC-3′	0.14	1,821
XF0318	nuoN	NADH-ubiquinone oxidoreductase, NQO14 subunit	Aerobic respiration	5′-GGGTTAAACATTGCCGATCT-3′, 5′-CGGGTTCCAAAGGATTCCTAA-3′	0.14	1,311
XF0656	gltT	Glutamate symport protein	Transport, amino acids	5′-TTGGGTGTGGGTACGTTGCTG-3′, 5′-CGCTGCCTCGTAAACCGTTGT-3′	0.09	951
XF0832	cysG	Siroheme synthase	Biosynthesis, heme and porphyrin	5′-GGCGGCGGTAAGGTTG-3′, 5′-GCGTATGTCTGTGCGGTGTGC-3′	0.32	1,170
XF0910	petC	Ubiquinol cytochrome c oxidoreductase, cytochrome c1 subunit	Electron transport	5′-CTGCCATTCGTTGAAGTACCT-3′, 5′-CGTCCTCCCAATAAGCCT-3′	0.32	531
XF1632	pilU	Twitching motility protein (pilU)	Surface structures	5′-CAATGAAGATTCACGGCAATA-3′, 5′-ATAGTTAATGGCTCCGCTATG-3′	0.17	873
XF1818	leuA	2-Isopropylmalate synthase (leuA)	Amino acid biosynthesis	5′-GGGCGTAGACATTATCGAGAC-3′, 5′-GTATCGTTGTGGCGTACACTG-3′	0.08	1,218
XF2447	lacF	ABC transporter sugar permease (lacF)	Transport, carbohydrates	5′-TTGCTGGTCCTGCGGTGTTG-3′, 5′-CCTCGGGTCATCACATAAGGC-3′	0.15	642

^aGene position ID, name, and function are based on those reported in reference 37.

^bK_A/K_S calculated across all N. American isolates.