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. 2005 Jul;71(7):3642–3652. doi: 10.1128/AEM.71.7.3642-3652.2005

FIG. 1.

FIG. 1.

Halo formation on CGP overlay agar plates caused by extracellular enzymes of S. hongkongensis strain KI and cell morphology of strain KI and C. amalonaticus strain G. After 14 days of incubation of S. hongkongensis strain KI on CGP-containing agar plates under a nitrogen atmosphere, a faint diffuse clear zone occurred around the inoculation spot (spot 1 in panel A); the spot became much clearer and appeared to be much more extended after staining of the remaining CGP with Serva Blue R (spot 1 in panel B). C. hydroxybenzoicum DSMZ 7310 was used as a reference strain and did not degrade CGP (spot 2 in panels A and B [inoculation spots are indicated by arrows]). C. hydroxybenzoicum medium containing 0.2% (wt/vol) CGP in the upper layer was used as the basic medium for cultivation experiments on agar plates. The light micrograph in panel C shows pairs of curved vegetative cells of S. hongkongensis strain KI which grew at very low cell densities in CGP-containing liquid medium. (D) One cell of strain KI exhibiting a typical terminal endospore. (E) Light micrograph of the straight, rod-shaped cells of C. amalonaticus strain G grown on solid LB medium.