FIG. 8.

Formation of ethanol and acetic acid as fermentation products of S. hongkongensis strain KI cultivated in CGP-containing Pelobacter liquid medium under anaerobic conditions. The samples analyzed were collected from the cultures used for the experiments whose results are shown in Fig. 5, and the same sampling times were used. Ethanol (A) and acetic acid (B) were the only secondary CGP degradation products occurring in significant amounts, as detected by gas chromatography. Two cultures (□ and ▵) also contained 0.01% yeast extract, and two cultures (▪ and ▴) were not supplemented with complex compounds. The time of inoculation of the cultures with C. amalonaticus strain G cells is indicated by arrowheads. Sharp increases in the ethanol concentration and the acetic acid concentration were observed after the addition of C. amalonaticus at 30 days.