TABLE 2.
Phenanthrene mineralization in NSP and HASP cultures during a 7-day incubationa
| Strainc | Enrichment approachd | % 14Cb
|
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|---|---|---|---|---|---|---|---|
| NSP
|
HASP
|
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| CO2 | Solution | Total | CO2 | Solution | Total | ||
| Ch1-1 | HASP | 70 ± 0 | 24 ± 0 | 98 ± 1 | 67 ± 16* | 49 ± 19 | 115 ± 3 |
| Ch3-5 | HASP | 75 ± 1 | 16 ± 4 | 91 ± 1 | 76 ± 6* | 37 ± 7 | 113 ± 8 |
| Eh1-1 | HASP | 67 ± 3 | 26 ± 4 | 94 ± 3 | 77 ± 5* | 31 ± 6 | 108 ± 9 |
| Eh2-1 | HASP | 28 ± 7 | 57 ± 2 | 86 ± 7 | 38 ± 10* | 72 ± 12 | 110 ± 15 |
| Chnp3-1 | HAB | 59 ± 1 | 33 ± 1 | 92 ± 1 | 1 ± 0 | 102 ± 2 | 103 ± 3 |
| Chnp3-4 | HAB | 56 | 31 | 87 | 1 ± 0 | 98 ± 0 | 98 ± 0 |
| Chnp3-5 | HAB | 61 ± 0 | 26 ± 7 | 87 ± 6 | 1 ± 0 | 104 ± 4 | 105 ± 4 |
| As1-2 | NSP | 66 | 27 | 93 | 1 ± 0 | 106 ± 2 | 106 ± 2 |
| As1-3 | NSP | 18 ± 1 | 65 ± 5 | 82 ± 3 | 1 ± 0 | 103 ± 5 | 104 ± 5 |
| As1-5 | NSP | 76 | 19 | 95 | 1 ± 0 | 104 ± 7 | 105 ± 7 |
| As1-6 | NSP | 34 | 61 | 86 | 1 ± 0 | 110 ± 4 | 111 ± 4 |
| As2-1 | NSP | 15 | 69 | 84 | 1 ± 0 | 107 ± 0 | 108 ± 0 |
| As2-4 | NSP | 64 | 27 | 91 | 1 ± 0 | 103 ± 1 | 103 ± 1 |
| As3-4 | NSP | 70 ± 1 | 26 ± 1 | 96 ± 1 | 3 ± 0 | 106 ± 0 | 109 ± 1 |
| Cs1-4 | NSP | 65 ± 1 | 28 ± 2 | 93 ± 3 | 2 ± 0 | 104 ± 1 | 105 ± 1 |
| Cs3-2 | NSP | 68 ± 0 | 22 ± 1 | 90 ± 1 | 1 ± 0 | 106 ± 1 | 107 ± 1 |
| Es2-1 | NSP | 24 | 45 | 70 | 4 ± 7 | 94 ± 8 | 99 ± 7 |
| Mc1 | NSP | 63 ± 1 | 29 ± 1 | 91 ± 1 | 2 ± 1 | 90 ± 1 | 92 ± 2 |
| Mc2 | NSP | 33 ± 14 | 38 ± 5 | 71 ± 8 | 3 ± 3 | 92 ± 2 | 95 ± 6 |
| Mc4 | NSP | 18 ± 8 | 45 ± 22 | 63 ± 12 | 2 ± 2 | 90 ± 5 | 93 ± 3 |
| Mc5 | NSP | 59 | 23 | 81 | 1 ± 1 | 100 ± 17 | 102 ± 16 |
| Mc6 | NSP | 69 ± 4 | 25 ± 3 | 93 ± 1 | 1 ± 0 | 108 ± 4 | 108 ± 4 |
| Sg5 | NSP | 71 | 21 | 93 | 1 ± 0 | 104 ± 6 | 104 ± 6 |
| Sg6 | NSP | 67 ± 1 | 26 ± 1 | 93 ± 1 | 1 ± 0 | 107 ± 4 | 108 ± 4 |
| Um1 | NSP | 62 ± 1 | 28 ± 2 | 90 ± 3 | 1 ± 0 | 89 ± 5 | 89 ± 5 |
| P5-2 | Outside | 57 ± 13 | 23 ± 5 | 81 ± 8 | 1 ± 0 | 68 ± 6 | 70 ± 5 |
| F199 | Outside | 63 ± 4 | 28 ± 4 | 91 ± 0 | 4 ± 5 | 72 ± 17 | 77 ± 12 |
| Abiotic | NA | 5 ± 3 | 61 ± 11 | 66 ± 10 | 1 ± 0 | 98 ± 7 | 99 ± 7 |
NSP contained a total of 14 μg ml−1 phenanthrene in MSM. HASP contained a total of 14 μg ml−1 phenanthrene in MSM with 2% (wt/vol) humic acid.
% 14C, percentage of total added 14C recovered in the indicated fraction. For HASP isolates, values are means from four replicates ± standard deviations. For all other isolates, values are means from duplicated tests ± standard deviations. Some NSP tests had poor mass balances (<70%); these were excluded from the table, and results from the single remaining test are presented without a standard deviation. All NSP percent CO2 means were significantly different from the abiotic control (Dunnett's method; α = 0.05). None of the HASP percent CO2 means were significantly different from the abiotic control (Dunnett's method; α = 0.05). Asterisks indicate that NSP percent CO2 means were significantly different from those for the abiotic control (Dunnett's method; α = 0.05). Percent CO2 means in NSP and HASP cultures were not significantly different (Wilcoxon rank sum test; α = 0.05).
See Table 1 for further information on strain identities. Strains listed in Table 1 but not included in the above data set because of inactive inocula were Cs1-5, MC3, Ps1, Es2-1, 12H6, As2-1, Es2-5, and Xan. Abiotic, noninoculated controls of NSP and HASP cultures.
“Outside” indicates an isolate acquired from an outside source. NA, not applicable.