TABLE 1.
Features of phage 2972 ORFs and the putative functions of their products
| ORF | Start | Stop | Size (aa) | Mol mass (kDa) | pI | Putative function and motifa | Best match(es) (% amino acid identity) |
|---|---|---|---|---|---|---|---|
| 1 | 414 | 827 | 137 | 16.1 | 8.7 | — | ORF137 S. thermophilus phage Sfi11 (135/137; 98%) |
| 2 | 1009 | 1461 | 150 | 16.7 | 8.0 | Small terminase | ORF25 S. thermophilus phage O1205 (74/147; 50%) |
| 3 | 1448 | 2119 | 223 | 25.3 | 9.0 | Large terminase | ORF411 phage Sfi11 (211/217; 97%) |
| 4 | 2404 | 2991 | 195 | 22.6 | 4.8 | Large terminase | ORF411 phage Sfi11 (183/192; 95%) |
| 5 | 3000 | 4505 | 501 | 57.4 | 5.0 | Portal protein | ORF27 phage O1205 (472/501; 94%) |
| 6 | 4502 | 5395 | 297 | 34.3 | 8.8 | Capsid protein | ORF28 phage O1205 (286/297; 96%) |
| 7 | 5583 | 6164 | 193 | 21.2 | 4.8 | Scaffold protein | ORF29 phage O1205 (184/193; 95%) |
| 8 | 6184 | 6543 | 119 | 12.7 | 7.9 | Capsid protein | ORF119 phage Sfi11 (110/119; 92%) |
| 9 | 6562 | 7608 | 348 | 37.4 | 4.9 | Capsid protein | ORF348 phage Sfi11 (326/348; 93%) |
| 10 | 7620 | 7781 | 53 | 5.9 | 9.3 | — | ORF32 phage O1205 (46/53; 86%) |
| 11 | 7793 | 8134 | 113 | 13.0 | 4.6 | — | ORF33 phage O1205 (108/112; 96%) |
| 12 | 8131 | 8445 | 104 | 11.4 | 9.5 | — | ORF34 phage O1205 (88/104; 84%) |
| 13 | 8447 | 8785 | 112 | 12.4 | 8.9 | — | ORF114 phage Sfi11 (94/112; 83%) |
| 14 | 8787 | 9173 | 128 | 14.6 | 5.0 | — | ORF128 phage Sfi11 (116/128; 90%) |
| 15 | 9187 | 9696 | 169 | 18.5 | 4.9 | Tail protein | ORF37 phage O1205 (157/169; 92%) |
| 16 | 9772 | 10125 | 117 | 13.1 | 5.0 | — | ORF117 phage Sfi11 (114/117; 97%) |
| 17 | 10176 | 10493 | 105 | 12.6 | 9.9 | — | ORF105 phage Sfi11 (103/105; 98%) |
| 18 | 10483 | 15036 | 1517 | 153.5 | 9.5 | TMP | ORF1510 phage Sfi11 (1191/1523; 78%) |
| 19 | 15036 | 16571 | 511 | 57.7 | 5.2 | Tail protein | ORF17 S. thermophilus phage DT1 (282/449; 62%), ORF512 phage Sfi11 (298/518; 57%), ORF41 phage O1205 (297/518; 57%), ORF515 S. thermophilus phage Sfi21 (264/448; 58%), ORF515 S. thermophilus phage Sfi19 (261/448; 58%), ORF34 S. thermophilus phage 7201 (186/455; 40%) |
| 20 | 16571 | 21388 | 1605 | 177.3 | 5.3 | Receptor-binding protein | ORF18 S. thermophilus phage MD2 (540/800; 67%) |
| 21 | 21389 | 23410 | 673 | 74.2 | 6.1 | Tail protein | ORF46 phage O1205 (462/674; 68%), ORF669 phage Sfi11 (463/674; 68%), ORF670 phage Sfi21 (392/676; 57%), ORF39 phage 7201 (391/676; 57%), ORF670 phage Sfi19 (391/676; 57%), ORF19 phage DT1 (345/663; 52%) |
| 22 | 23427 | 23813 | 128 | 14.5 | 4.6 | — | ORF149 phage Sfi11 (83/123; 67%), ORF21 phage DT1 (81/114; 71%), ORF131 phage Sfi19 (80/114; 70%), ORF47 phage O1205 (78/112; 69%), ORF117 phage Sfi21 (74/112; 66%), ORF40 phage 7201 (75/126; 59%) |
| 23 | 23839 | 23982 | 47 | 5.4 | 6.6 | — | S. pyogenes prophage 315.5 (24/40; 60%) |
| 24 | 24009 | 24341 | 110 | 12.3 | 5.0 | — | No hit |
| 25 | 24366 | 24692 | 108 | 12.0 | 5.5 | Holin | S. pyogenes prophage 315.5 (63/104; 60%), S. mitis phage SM1 (59/107; 55%), S. pneumoniae phage MM1 (58/106; 54%) |
| 26 | 24689 | 25288 | 199 | 21.7 | 4.8 | Endolysin | ORF25 phage DT1 (162/194; 83%), ORF44 phage 7201 (160/192; 83%), ORF288 phage Sfi19 (158/193; 81%), ORF288 phage Sfi21 (158/193; 81%), ORF288 phage Sfi11 (157/193; 81%), ORF51 phage O1205 (151/193; 78%), |
| 27 | 25334 | 25456 | 40 | 4.5 | 8.8 | — | No hit |
| 28 | 25495 | 25680 | 61 | 6.8 | 9.4 | Endonuclease | S. thermophilus phage S3b (54/54; 100%), S. thermophilus phage ST3 (54/54; 100%), ORF26 phage DT1 (60/61; 98%) |
| 29 | 25747 | 25974 | 75 | 8.6 | 4.1 | Endolysin | ORF44 phage 7201 (65/75; 86%), ORF51 phage O1205 (62/75; 82%), ORF288 phage Sfi11 (59/75; 78%), ORF288 phage Sfi21 (59/75; 78%), ORF288 phage Sfi19 (57/75; 76%) |
| 30 | 26142 | 26273 | 43 | 5.2 | 8.9 | — | No hit |
| 31 | 26374 | 26583 | 69 | 7.8 | 7.9 | cro-like repressor | ORF69 phage Sfi19 (67/69; 97%) |
| 32 | 26600 | 26722 | 40 | 5.0 | 8.0 | — | ORF40 phage Sfi11 (40/40; 100%) |
| 33 | 26966 | 27439 | 157 | 18.0 | 6.2 | — | ORF157 phage Sfi19 (157/157; 100%) |
| 34 | 27436 | 28137 | 233 | 26.1 | 6.6 | SSAP | ORF9 phage O1205 (89/235; 37%) |
| 35 | 28094 | 29431 | 445 | 50.9 | 8.8 | Helicase | ORF443 phage Sfi21 (383/441; 86%) |
| 36 | 29438 | 29893 | 151 | 17.2 | 4.9 | — | ORF151 phage Sfi19 (150/151; 99%) |
| 37 | 29896 | 30711 | 271 | 30.4 | 5.8 | Replication protein | ORF271 phage Sfi21 (264/271; 97%) |
| 38 | 30698 | 32215 | 505 | 59.4 | 8.1 | Primase | ORF504 phage Sfi11 (457/504; 90%) |
| 39 | 32466 | 32786 | 106 | 12.1 | 9.9 | — | ORF106 phage Sfi11 (103/105; 98%) |
| 40 | 32770 | 33021 | 83 | 9.5 | 8.0 | — | ORF89 phage Sfi19 (61/78; 78%) |
| 41 | 33029 | 33184 | 51 | 6.3 | 5.6 | — | ORF40 phage DT1 (45/51; 88%) |
| 42 | 33185 | 33697 | 170 | 19.5 | 6.3 | DNA binding | ORF42 phage DT1 (117/166; 70%) |
| 43 | 33666 | 33992 | 108 | 12.1 | 9.2 | — | ORF43 phage DT1 (55/84; 65%) |
| 44 | 33996 | 34703 | 235 | 27.6 | 9.1 | — | ORF235 phage Sfi19 (230/235; 97%) |
a
—, unknown function; TMP, tape measure protein; SSAP, single-strand annealing protein.