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SDS-PAGE analysis of purified B. breve UCC2003 β-fructofuranosidase. Proteins were denatured, separated on a 12.5% polyacrylamide gel, and stained with Coomassie blue R250. Lane M, molecular mass marker; lanes L1 to L4, lysate fractions; lanes W1 to W4, wash fractions; lane E1, purified β-fructofuranosidase.
