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Inhibition of Cry1Aa binding to BmAPN1 by monoclonal antibodies. Biotinylated Cry1Aa was preincubated with or without each MAb at a concentration of 500 nM for 90 min and then added to wells coated with 7-kDa BmAPN1. Then it was incubated for 90 min with horseradish peroxidase-conjugated streptavidin. Bound streptavidin was detected after incubation with an ABTS solution for 15 min by determining the optical density at 415 nm using a microtiter plate reader.
