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Dose-dependent inhibition of Cry1Aa binding to BmAPN1 by blocking antibodies. Biotinylated Cry1Aa was preincubated with various concentrations of intact MAbs and Fab fragments of MAbs for 90 min and then added to wells coated with 7-kDa BmAPN1. Then it was incubated for 90 min with horseradish peroxidase-conjugated streptavidin. Bound streptavidin was detected after incubation with an ABTS solution for 15 min by determining the optical density at 415 nm using a microtiter plate reader.
