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Determination of binding compatibility using the IAsys optical sensor. The tests were performed using Cry1Aa covalently immobilized on the carboxylated surface of a cuvette and the IAsys optical sensor. The molecules were injected successively. (A) First MAb 1B10 was added to block its epitope on the immobilized Cry1Aa, and then MAbs 1B10, 2F9, and 2C2 were added. (B) First MAb 2F9 was added to block its epitope on the immobilized Cry1Aa, and then MAbs 1B10, 2F9, and 2C2 were added.
