FIG. 7.

Epitopes of blocking antibodies in a three-dimensional model of Cry1Aa. (A) The amino acid residues in domains I, II, and III are pink, light green, and light blue, respectively. The single octapeptide to which MAb 1B10 bound, ⁵⁸²VFTLSAHV⁵⁸⁹, is red. The three octapeptides to which MAb 2C2 bound, ⁵²⁰QRYRVRIR⁵²⁷, ⁵⁷⁰FTTPFNFS⁵⁷⁷, and ⁵⁸²VFTLSAHV⁵⁸⁹ are blue, magenta, and red, respectively. In addition, Arg521 (green) and Val582 (yellow), each of which was mutated to cysteine, are shown. The peptide ⁵⁰⁸STLRVN⁵¹³ (brown) adjoins the ⁵⁸²VFTLSAHV⁵⁸⁹ site in the three-dimensional structure; this region is expected to be involved in the binding of the blocking MAbs. The candidate binding sites for MAbs 1B10 and 2C2 are indicated by blue and red circles. (B) Panel A rotated 72° about the y axis and 45° about the x axis. (C) Panel A rotated 90° about the x axis.