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. 2005 Jul;71(7):3966–3977. doi: 10.1128/AEM.71.7.3966-3977.2005

FIG. 9.

FIG. 9.

Immunodot blot analysis with MAbs 3C7, 1G10, 1B10, 2C2, 1E10, 2F9, and, 2A11 using Cry1Aa, Cry1Ac, R521C, R521C-NAM, V582C, and V582C-NAM as the antigens. The following six toxin constructs were dotted onto a nitrocellulose membrane at a concentration of 100 ng/μl: Cry1Aa (spot A), Cry1Ac (spot B), R521C (spot C), R521C-NAM (spot D), V582C (spot E), and V582C-NAM (spot F). These constructs were probed with the following seven horseradish peroxidase-conjugated MAbs: 3C7 (blot a) 1G10 (blot b), 1B10 (blot c), 2C2 (blot d), 1E10 (blot e), 2F9 (blot f), and 2A11 (blot g). Bound MAb was detected using the ECL system.