TABLE 1.
Immunoreactivity of MAbs against wild-type (N615) and deletion mutant proteins of Cry1Aa
| Cry1Aa constructa | Reactivity of MAbsb
|
||||||
|---|---|---|---|---|---|---|---|
| 3C7 | 1G10 | 1B10 | 2C2 | 1E10 | 2F9 | 2A11 | |
| C-terminal deletions | |||||||
| N615 | + | + | + | + | + | + | + |
| N593 | + | + | + | + | − | − | − |
| N570 | + | + | + | + | − | − | − |
| N531 | + | + | + | + | − | − | − |
| N513 | + | + | + | + | − | − | − |
| N507 | + | + | − | − | − | − | − |
| N282 | + | + | − | − | − | − | − |
| N-terminal deletions | |||||||
| C197 | − | + | + | + | + | − | − |
| C255 | − | + | + | + | + | − | − |
| C354 | − | − | + | + | + | − | − |
| C456 | − | − | +c | +c | + | − | − |
| C514 | − | − | +c | +c | + | − | − |
a
Cry1Aa deletion mutants are illustrated in Fig. 5A.
b
+, immunoreactive; −, not immunoreactive.
c
These analyses were performed after thrombin cleavage of GST fusion deletion mutant proteins.