TABLE 2.

Transformation of T. kodakaraensis using various DNAs^a

^aTransformation was performed without CaCl₂, and outgrowth was carried out for 2 h. The order of magnitude of transformation efficiency is the mean obtained from three independent experiments. The circular plasmids were prepared using an E. coli DH5α strain harboring a methylation system, while the linear DNAs were prepared by PCR without methylation.