TABLE 3.
Subtyping of stx2 genes by RFLP
| Primer (size in bp)b | Enzyme | Fragment lengths (bp) in STEC strain (subtype designation):
|
|||||
|---|---|---|---|---|---|---|---|
| 43894 (VT2) | W1 (VT2vh-a) | W2 (VT2vh-a) | SH1 (VT2vh-a) | SH2 (VT2vh-a) | SH3 (VT2vh-a) | ||
| Lin FRa (900) | HincII | 750 | 550 | 550 | 550 | 550 | 550 |
| 150 | 350 | 350 | 350 | 350 | 350 | ||
| VT2efc (348) | HeaII | 348 | 216 | 216 | 216 | 216 | 216 |
| 132 | 132 | 132 | 132 | 132 | |||
| PvuII | 323 | 323 | 323 | 323 | 323 | 323 | |
| 25d | 25d | 25d | 25d | 25d | 25d | ||
| Tylere (285) | RsaI | 216 | 136 | 136 | 136 | 136 | 136 |
| 69d | 80 | 80 | 80 | 80 | 80 | ||
| 69d | 69d | 69d | 69d | 69d | |||
a
This primer pair was used according to the method of Lin et al. (30).
b
Expected size of amplified product.
c
This primer pair was used according to the method of Pierard et al. (39).
d
Fragments did not resolve or were too small to be clearly visible under the electrophoresis conditions used.
e
This primer pair was used according to the method of Tyler et al. (48).