TABLE 1.

Characteristics of initially discrepant EV-positive samples

Patient no.	Age	Sample	Result by:				CSF profile	Culture result	Clinical findings
			NASBA-ECLa		NASBA-beacon
			Initial	Repeat	Initial	Repeat
1	6 yr	CSF	7,959	6,691	Indeterminate	Positive	Abnormal	Negative	Aseptic meningitis
2	10 wk	CSF	3,701	Not done	Indeterminate	Positive	Normal	Negative	Fever, irritability
3	15 yr	Stool	1,179,369	26,863	Indeterminate	Indeterminate	Not done	Negativeb	Fever, cough, rhinorrhea, vomiting, dizziness
4	17 yr	CSF	Invalid	1,271	Indeterminate	Negative	Abnormalc	Negative	Fever, headache, neck stiffness, photophobia
5	9 wk	CSF	2,937	651	Negative	Negative	Abnormal	EV isolated from CSF and rectal swab	Fever, vomiting, loose stools

^aExpressed in ECL units, with ≥650 considered positive.

^bThe stool sample was also positive for EV by nested RT-PCR at CDC; the amplicon sequenced as coxsackievirus A1.

^cCSF profile: 940 nucleated cells, 13% granulocytes, 72% lymphocytes, 4% monocytes; protein, 103 mg/dl; glucose, 64 mg/dl; sample collected on day 5 of illness.