TABLE 2.
Sensitivities and specificities of PCR assays
| PCR primer pair | Target | No. of PCR-positive strains/no. of tested strains
|
Sensitivity (%) | Specificity (%) | ||
|---|---|---|---|---|---|---|
| Ralstonia spp.a | R. respiraculib | Otherc | ||||
| RalGS-F and RalGS-R | All Ralstonia species | 79/79 | 13/13 | 0/60 | 100 | 100 |
| Rres-F and Rres-R | Ralstonia respiraculi | 0/24 | 13/13 | 0/16 | 100 | 100 |
a
Includes R. basilensis, R. campiensis, R. eutropha, R. gilardii, R. metallidurans, R. oxalatica, R. paucula, R. solanacearum, and R. taiwanensis isolates from the BCCM/LMG culture collection and R. gilardii, R. insidiosa, R. mannitolilytica, R. pickettii, R. respiraculi, and an unidentified Ralstonia sp. recovered from CF sputum cultures (see the text).
b
CF sputum isolates identified by polyphasic testing, including 16S rRNA gene sequence analysis.
c
“Other” includes strains representing 23 non-Ralstonia species.