TABLE 1.
Primers and probes used for detection of meningeal pathogens by PCR
| Target organism | Primer pair | Probes | Reference(s) |
|---|---|---|---|
| Staphylococcus spp. | RW01/DG74 | Staph FL/LC | 5, 30 |
| Staphylococcus aureus | Sa442-F/Sa442-R | Sa442-HP-1/-2 | 20 |
| Staphylococcus epidermidis | MasFs/epiR1 | Epi FL/LC | 30 |
| Streptococcus agalactiae | Sag59/Sag190 | STB-F-HP-1/-2 | 10, 30 |
| Streptococcus spp. | RW01/DG74 | EntFL/LCa | 30 |
| Enterococcus spp. | RW01/DG74 | EntFL/LCa | 30 |
| Neisseria meningitidis | MenIS-1/-8 | MenIS_HP-1/-4 | 30 |
a
Differentiation of Streptococcus spp. and Enterococcus spp. was done by melting curve analysis, as described in Materials and Methods.