TABLE 2.
Probes used for detection of meningeal pathogens by FISH
| Target organism | Probea | Type | % [FA]b | Sequence (5′-3′) | Reference |
|---|---|---|---|---|---|
| Escherichia coli | Esco 473 | DNA | 30 | GCG GGT AAC GTC AAT GAG C | This study |
| Haemophilus influenzae | Haeinf | DNA | 30 | CCG CAC TTT CAT CTT CCG | 7 |
| Neisseria meningitidis | NeMe183c | DNA | 30 | CCT GCT TTC TCT CTC AAG A | This study |
| S. agalactiae | Saga | DNA | 20 | GTA AAC ACC AAA CMT CAG CG | 27 |
| S. pneumoniae | Spn | DNA | 20 | GTG ATG CAA GTG CAC CTT | 12 |
| Staphylococcus spp. | Staph 698 | PNA | 30 (PNA mix) | CTC CAT ATC TCT GCG | This study |
| S. aureus | Stau | PNA | 30 (PNA mix) | GCT TCT CGT CCG TTC | 17 |
| Bacterial kingdom | EUB 338 | DNA | 20-40 | GCT GCC TCC CGT AGG AGT | 2 |
| Bac-Uni1 | PNA | 30 (PNA mix) | CTG CCT CCC GTA GGA | 17 |
a
All probes are directed to complementary sequences of the 16S subunit of the ribosome.
b
Concentration of formamide used in hybridization buffer. Additional substances were added for DNA-FISH as described in references 2,15 and for PNA FISH as described in reference 17.
c
To be used in combination with competitor (5′-CCTGCTTTCCCTCTCAAGA-3′).