TABLE 3.
Algorithm used for the FISH and PCR approaches
| Result of Gram stain | Organism(s) tested for by:
|
|
|---|---|---|
| PCR assays | FISH (probe type) | |
| Gram-positive cocci in clusters | Staphylococcus aureus | Staphylococcus aureus (PNA) |
| Staphylococcus spp. | Staphylococcus spp. (PNA) | |
| Staphylococcus epidermidis | ||
| Gram-positive cocci in chains and diplococci | Enterococcus/Streptococcus spp. | S. pneumoniae (DNA) |
| S. agalactiae | S. agalactiae (DNA) | |
| Gram-negative cocci | Neisseria meningitidis | Neisseria meningitidis (DNA) |
| Gram-negative rods | E. coli | E. coli (DNA) |
| Haemophilus influenzae | Haemophilus influenzae (DNA) | |
| No bacteria visible | Eubacteria | Eubacteria (PNA) |