TABLE 4.
Diagnostic sensitivity of FISH and PCR
Bacterial species isolated from CSF samples | No. of positive samples/total no. of samples tested by:
|
|
---|---|---|
FISH | PCR | |
Neisseria meningitidis | 1/2 | 4/4 |
Streptococcus pneumoniae | 4/4 | 7/7b |
Streptococcus agalactiae | 0/0 | 2/2 |
Staphylococcus aureus | 1/2 | 2/2 |
Staphylococcus epidermidis | 4/6a | 7/7 |
Coagulase-negative staphylococci other than S. epidermidis | 1/2a | 3/3 |
Enterococcus faecalis | 1/1c | 1/1d |
Bacillus spp. | 0/0 | 1/1c |
Corynebacterium spp. | 1/1c | 1/1c |
Total | 13/18 | 28/28 |
For coagulase-negative staphylococci, a genus-specific Staphylococcus FISH probe was implemented.
S. pneumoniae was identified as Streptococcus spp. by the Enterococcus/Streptococcus-specific PCR.
Positive with the eubacterial FISH probe or the eubacterial PCR, respectively.
Positive in the Enterococcus/Streptococcus-specific PCR and identified as E. faecalis by melting point analysis (30).