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. 2005 Jul;43(7):3390–3397. doi: 10.1128/JCM.43.7.3390-3397.2005

TABLE 4.

Diagnostic sensitivity of FISH and PCR

Bacterial species isolated from CSF samples No. of positive samples/total no. of samples tested by:
FISH PCR
Neisseria meningitidis 1/2 4/4
Streptococcus pneumoniae 4/4 7/7b
Streptococcus agalactiae 0/0 2/2
Staphylococcus aureus 1/2 2/2
Staphylococcus epidermidis 4/6a 7/7
Coagulase-negative staphylococci other than S. epidermidis 1/2a 3/3
Enterococcus faecalis 1/1c 1/1d
Bacillus spp. 0/0 1/1c
Corynebacterium spp. 1/1c 1/1c
Total 13/18 28/28
a

For coagulase-negative staphylococci, a genus-specific Staphylococcus FISH probe was implemented.

b

S. pneumoniae was identified as Streptococcus spp. by the Enterococcus/Streptococcus-specific PCR.

c

Positive with the eubacterial FISH probe or the eubacterial PCR, respectively.

d

Positive in the Enterococcus/Streptococcus-specific PCR and identified as E. faecalis by melting point analysis (30).