FIG. 1.

Amplification plots and standard curves. (A) Serial dilution of adenoviral DNA (species A, serotype 2) ranging from 10² to 10⁷ virus particles per reaction. The analysis was done in duplicate using the ACF RQ-PCR system. (B) Serial dilution of adenoviral DNA (species B, serotype 7) ranging from 10² to 10⁷ virus particles per reaction. The analysis was done in duplicate using the BDE system. The amplification plots displayed were concordant with those established using serial dilutions of serotypes representative of other AdV species. The amplification plots generated on the basis of the above serial dilutions therefore served as external standard curves for the ACF and BDE RQ-PCR detection systems, respectively.