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. 2024 Dec 4;136(1):91–111. doi: 10.1161/CIRCRESAHA.124.325187

Figure 1.

Figure 1.

GSK3 (glycogen synthase kinase-3) inhibition stimulates vasculature expansion in adipose tissue of obese mice. Male C57Bl/6J lean mice (LE) were fed a normal chow diet, whereas male obese mice (OB) were fed a high-fat diet with or without SB216763 treatment (OB+SB). A, Gene ontology analysis showing upregulated gene signatures in the adipose tissue of the OB+SB mice vs OB mice (n=2). B, Quantification of the expression of angiogenesis marker genes in epididymal white adipose tissue (eWAT) from indicated mice using reverse transcription polymerase chain reaction (n=6). C, Western blotting analysis of VEGF (vascular endothelial growth factor) and VEGFB in eWAT from the indicated mice (n=6). D, ELISA assay of VEGF protein levels in eWAT from the indicated mice (n=6). E through G, Representative confocal microscope images of whole-mount CD31 staining in the indicated eWAT (E) and analysis for capillary branch density (F) and CD31-positive area (G) using ImageJ software (n=6). Scale bar=100 µm. B, D, F, and G, One-way ANOVA and Tukey post hoc test. PIGF indicates placental growth factor.