Figure 11.

Confirmation of expression of MFAP5 in human aortic valve tissue. (A) Relative mRNA levels were detected by qPCR. (B) Immunofluorescence analysis showed increased MFAP5 expression in calcified aortic valve tissue. (C) Representative Western blot (WB) analysis and quantification of MFAP5 protein levels in human valve tissue. (D) Immunofluorescence staining of MFAP5 in aortic valves, scale bar: 100 μm. All statistical differences were determined using two-tailed unpaired Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.