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. Author manuscript; available in PMC: 2025 Jan 2.
Published in final edited form as: Brain Behav Immun Integr. 2024 Aug 23;8:100081. doi: 10.1016/j.bbii.2024.100081

Fig. 4. Microglia localization, size, and number differed among offspring of prenatally injected mothers.

Fig. 4.

(A) Proximity of microglia to blood vessels (within 1.18 μm), (B) average size of IBA-1-labeled microglia cells, and (C) the number of IBA-1-labeled cells in the PVN. (D) Representative images. VEH = vehicle, RQ = Resiquimod, NR = no restraint, R = restraint, PVN = Paraventricular Nucleus of the Hypothalamus, 3 V = 3rd ventricle, FITC = fluorescein isothiocyanate, IBA1 = ionizing binding calcium adapter molecule-1. *p < 0.05, **p < 0.01, ***p < 0.001, ****p<0.0001. Error bars represented as +/− SEM. n = 8–10 animals / group.