Virulence assay on Kalanchöe leaves. Wild-type (C58), Δ1392, Δ4851, Δ5306, Δ5307, and A136 (a negative control) strains were grown in MG/L media as described in Materials and Methods, and 1 μl of each culture was used to inoculate Kalanchöe leaves. Photos were taken 30 days after inoculation.