FIG. 4.

Functional complementation of A. tumefaciens CS in Sinorhizobium. The vectors, each containing one of the cloned putative CS genes, were introduced by electroporation into S. meliloti Δ1A, a mutant with a mutation in CS. The cells were plated on MM NH₄ medium with arabinose and kanamycin. Colonies were then streaked out onto MM NH₄± arabinose. Growth in the absence of arabinose indicates that the cell has a functional CS.