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(A) Key plasmids used in the generation of ST36 (ΔatpD::aphA-3). Allelic exchange delivery vector pST100 carries DNA amplified from the atp operon of M. smegmatis mc2155. The in trans ΔatpD complementing vector pST101 carries atpD under the constitutive control of the Mycobacterium bovis BCG Hsp60 promoter (Phsp60). (B) The genetic consequences of homologous recombination with ΔatpD::aphA-3 (see the text for detailed explanation).
