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Overexpression and purification of the E. coli YahA protein, the EAL domain of YahA, and the EAL domain of E. coli Dos (protein chip, Bioanalyzer). Lane 1, molecular mass markers (molecular masses [in kDa] are indicated on the left); lanes 2, 5, and 8, crude extracts of E. coli DH5α cells prior to induction of expression of the fusion proteins; lanes 3, 6, and 9, crude extracts of E. coli cells after 2 h of induction with IPTG; lanes 4, 7, and 10, purified recombinant proteins.
