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Expression of EngO in C. cellulovorans and E. coli. (A) Gels stained with Coomassie blue. (B) Gels stained for CMCase activity. Lanes: M, protein mass standards; 1, noncellulosomal proteins of C. cellulovorans; 2, supernatant of recombinant E. coli; 3, supernatant of knockout EngO recombinant E. coli. The oblong circles represent the putative EngO (79,474 Da).
