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Thin-layer chromatography of hydrolysis products from oligosaccharides and CMC. The oligosaccharides and CMC (1 mg) were incubated with enzyme (1 μg) for 16 h, and the hydrolysates were analyzed by thin-layer chromatography. S, authentic oligosaccharides; SC, CMC reaction mixture with knockout recombinant EngO; G1, glucose; G2, cellobiose; G3, cellotriose; G4, cellotetraose; G5, cellopentaose; C, CMC.
