TABLE 3.
Plasmid content | Relevant prg gene content | Exogenous pheromone-binding capacity |
---|---|---|
None | None | 0 |
pDL276 | None | 0 |
pMSP6049 | prgY | 0 |
pMSP6023ΔY | prgZ | 64 |
pMSP6043 | prgY prgZ | 64 |
E. faecalis OG1RF harboring the plasmids indicated was assayed for binding by assessing the ability of the bacteria to reduce the pheromone titer of a preparation of cCF10 (previously diluted to a titer of 64) by the assay described in Materials and Methods. The values listed for binding capacity indicate the reduction in pheromone titer observed after incubation of the pheromone with the bacterial strain indicated. This experiment was repeated twice in duplicate with the same results both times.