TABLE 2.
Summary of quorum sensing-related genes that were up-regulated in (i) isolate LES400 compared to strain PAO1, (ii) isolate LES431 compared to strain PAO1, and (iii) isolate LES431 compared to isolate LES400
| ORFa | Gene name | LB LES400 ↑ vs PAO1 | H2O2 LES400 ↑ vs PAO1 | LB LES431 ↑ vs PAO1 | H2O2 LES431 ↑ vs PAO1 | LB LES431 ↑ vs LES400 | H2O2 LES431 ↑ vs LES400 | Product description |
|---|---|---|---|---|---|---|---|---|
| QS activated | ||||||||
| PA0026 | 2.6 | 11 | Hypothetical protein | |||||
| PA0050 | 3.3 | Hypothetical protein | ||||||
| PA0059 | osmC | 4.6 | 6.3 | Osmotically inducible protein OsmC | ||||
| PA0106 | coxA | 64 | 26 | Cytochrome c oxidase, subunit 1 | ||||
| PA0107 | 24 | 21 | 5.0 | Conserved hypothetical protein | ||||
| PA0122 | 15 | 14 | Conserved hypothetical protein | |||||
| PA0143 | nuh | 11 | 24 | Nonspecific ribonucleoside hydrolase | ||||
| PA0176 | 3.1 | Probable chemotaxis transducer | ||||||
| PA0355 | pfpI | 2.8 | Protease PfpI | |||||
| PA0364 | 5.4 | 4.6 | Probable oxidoreductase | |||||
| PA0366 | 4.0 | 3.3 | Probable aldehyde dehydrogenase | |||||
| PA0447 | gcdH | 2.4 | Glutaryl-CoA dehydrogenase | |||||
| PA0567 | 16 | 9.2 | Conserved hypothetical protein | |||||
| PA0586 | 4.3 | 8.2 | Conserved hypothetical protein | |||||
| PA0588 | 5.2 | 6.9 | Conserved hypothetical protein | |||||
| PA0852 | cbpD | 44 | 55 | Chitin-binding protein CbpD precursor | ||||
| PA0996 | pqsA | 3.5 | 9.3 | 16 | Probable coenzyme A ligase | |||
| PA0997 | pqsB | 6.5 | 5.8 | 7.3 | Homologous to β-ketoacyl-acyl carrier protein synthase | |||
| PA0998 | pqsC | 4.6 | 6.5 | 5.0 | Homologous to β-ketoacyl-acyl carrier protein synthase | |||
| PA0999 | pqsD | 5.3 | 5.3 | 6.5 | 3-Oxoacyl-acyl carrier protein synthase III | |||
| PA1000 | pqsE | 6.2 | Quinolone signal response protein | |||||
| PA1001 | phnA | 12.1 | 4.6 | Anthranilate synthase component I | ||||
| PA1173 | napB | 5.6 | Cytochrome c-type protein NapB precursor | |||||
| PA1174 | napA | 3.5 | 4.4 | Periplasmic nitrate reductase protein NapA | ||||
| PA1175 | napD | 3.0 | 5.0 | NapD protein of periplasmic nitrate reductase | ||||
| PA1176 | napF | 6.4 | Ferredoxin protein NapF | |||||
| PA1177 | napE | 5.8 | 4.4 | Periplasmic nitrate reductase protein NapE | ||||
| PA1247 | aprE | 7.5 | Alkaline protease secretion protein AprE | |||||
| PA1248 | aprF | 12 | Alkaline protease secretion outer membrane protein AprF precursor | |||||
| PA1250 | aprI | 16 | 7.5 | Alkaline proteinase inhibitor AprI | ||||
| PA1323 | 11 | 6.7 | Hypothetical protein | |||||
| PA1324 | 7.5 | 5.3 | 3.3 | 5.8 | Hypothetical protein | |||
| PA1404 | 16.9 | 4.1 | 3.8 | 3.6 | Hypothetical protein | |||
| PA1431 | rsaL | 20 | 14 | 655 | 16 | Regulatory protein RsaL | ||
| PA1432 | lasI | 21 | Autoinducer synthesis protein LasI | |||||
| PA1656 | 3.3 | Hypothetical protein | ||||||
| PA1657 | 14 | 11 | Conserved hypothetical protein | |||||
| PA1658 | 4.4 | 24 | Conserved hypothetical protein | |||||
| PA1660 | 11 | Hypothetical protein | ||||||
| PA1662 | 15 | Probable ClpA/B-type protease | ||||||
| PA1667 | 12 | Hypothetical protein | ||||||
| PA1784 | 3.6 | 3.6 | 5.9 | Hypothetical protein | ||||
| PA1869 | 78 | 28 | 68 | Probable acyl carrier protein | ||||
| PA1871 | lasA | 4.9 | 5.2 | 12 | LasA protease precursor | |||
| PA1874 | 5.3 | Hypothetical protein | ||||||
| PA1881 | 31 | 3.8 | Probable oxidoreductase | |||||
| PA1891 | 5.9 | Hypothetical protein | ||||||
| PA1893 | 6.2 | Hypothetical protein | ||||||
| PA1894 | 3.4 | 11 | 4.8 | 6.1 | Hypothetical protein | |||
| PA1895 | 8.9 | 3.8 | Hypothetical protein | |||||
| PA1896 | 11 | Hypothetical protein | ||||||
| PA1897 | 4.2 | 13 | 24 | Hypothetical protein | ||||
| PA1901 | phzC2 | 38 | 126 | Phenazine biosynthesis protein PhzC | ||||
| PA1902 | phzD2 | 49 | 77 | Phenazine biosynthesis protein PhzD | ||||
| PA1903 | phzE2 | 31 | 35 | Phenazine biosynthesis protein PhzE | ||||
| PA1904 | phzF2 | 86 | 91 | Probable phenazine biosynthesis protein | ||||
| PA1905 | phzG2 | 22 | 70 | Probable pyridoxamine 5′-phosphate oxidase | ||||
| PA1999 | 2.8 | 2.6 | Probable CoA transferase, subunit A | |||||
| PA2000 | 2.9 | 2.8 | Probable CoA transferase, subunit B | |||||
| PA2001 | atoB | 3.5 | 2.5 | Acetyl-CoA acetyltransferase | ||||
| PA2030 | 15 | 10 | Hypothetical protein | |||||
| PA2031 | 6.2 | 10 | Hypothetical protein | |||||
| PA2066 | 6.0 | 4.4 | Hypothetical protein | |||||
| PA2067 | 8.5 | 11 | Probable hydrolase | |||||
| PA2068 | 20 | 18 | Probable MFS transporter | |||||
| PA2069 | 25 | 45 | Probable carbamoyl transferase | |||||
| PA2080 | 5.4 | Hypothetical protein | ||||||
| PA2143 | 33 | Hypothetical protein | ||||||
| PA2146 | 29 | 13 | 18 | 14 | Conserved hypothetical protein | |||
| PA2157 | 4.1 | Hypothetical protein | ||||||
| PA2159 | 13 | 3.8 | 12 | Conserved hypothetical protein | ||||
| PA2165 | 5.4 | 5.0 | Probable glycogen synthase | |||||
| PA2166 | 6.5 | 117 | 7.0 | 18 | Hypothetical protein | |||
| PA2171 | 14 | 5.4 | 12 | Hypothetical protein | ||||
| PA2172 | 6.5 | 3.2 | Hypothetical protein | |||||
| PA2180 | 2.3 | Hypothetical protein | ||||||
| PA2190 | 16 | 25 | Conserved hypothetical protein | |||||
| PA2193 | hcnA | 58 | 35 | Hydrogen cyanide synthase HcnA | ||||
| PA2194 | hcnB | 15 | 111 | Hydrogen cyanide synthase HcnB | ||||
| PA2195 | hcnC | 12 | 40 | Hydrogen cyanide synthase HcnC | ||||
| PA2274 | 4.1 | 2.6 | 6.7 | Hypothetical protein | ||||
| PA2300 | chiC | 7.7 | 7.0 | Chitinase | ||||
| PA2305 | 9.4 | 23 | Probable nonribosomal peptide synthetase | |||||
| PA2328 | 4.3 | Hypothetical protein | ||||||
| PA2329 | 4.9 | Probable ATP-binding component of ABC transporter | ||||||
| PA2330 | 2.5 | Hypothetical protein | ||||||
| PA2331 | 3.6 | Hypothetical protein | ||||||
| PA2345 | 2.8 | Conserved hypothetical protein | ||||||
| PA2365 | 32 | 12 | 7.3 | Conserved hypothetical protein | ||||
| PA2366 | 13 | 7.7 | 6.3 | 13 | Conserved hypothetical protein | |||
| PA2367 | 15 | 6.2 | Hypothetical protein | |||||
| PA2423 | 5.6 | Hypothetical protein | ||||||
| PA2433 | 15 | 6.9 | 2.9 | 2.8 | Hypothetical protein | |||
| PA2512 | antA | 7.6 | Anthranilate dioxygenase large subunit | |||||
| PA2513 | antB | 18 | 50 | Anthranilate dioxygenase small subunit | ||||
| PA2552 | 2.2 | Probable acyl-CoA dehydrogenase | ||||||
| PA2553 | 2.7 | Probable acyl-CoA thiolase | ||||||
| PA2587 | pqsH | 5.7 | 28 | Probable FAD-dependent mono-oxygenase | ||||
| PA2588 | 26 | 22 | Probable transcriptional regulator | |||||
| PA2591 | vqsR | 6.0 | 12 | Probable transcriptional regulator | ||||
| PA2592 | 4.3 | 5.4 | 4.9 | Probable periplasmic spermidine/putrescine-binding protein | ||||
| PA2747 | 6.6 | 5.4 | Hypothetical protein | |||||
| PA2939 | 147 | 134 | Probable aminopeptidase | |||||
| PA3032 | snr1 | 10 | 5.9 | Cytochrome c Snr1 | ||||
| PA3104 | xcpP | 3.7 | Secretion protein XcpP | |||||
| PA3181 | 5.4 | 3.5 | 10 | 2-Keto-3-deoxy-6-phosphogluconate aldolase | ||||
| PA3182 | pgl | 5.7 | 3.8 | 4.8 | 6-Phosphogluconolactonase | |||
| PA3183 | zwf | 6.2 | 2.9 | 11 | Glucose-6-phosphate 1-dehydrogenase | |||
| PA3326 | 14 | 11 | 14 | Probable Clp family ATP-dependent protease | ||||
| PA3327 | 5.6 | Probable nonribosomal peptide synthetase | ||||||
| PA3328 | 6.1 | 9.1 | 8.1 | Probable FAD-dependent mono-oxygenase | ||||
| PA3329 | 37 | 34 | 33 | Hypothetical protein | ||||
| PA3330 | 34 | 46 | 55 | Probable short-chain dehydrogenase | ||||
| PA3331 | 6.1 | 16 | 8.6 | Cytochrome P450 | ||||
| PA3332 | 26 | 30 | 13 | Conserved hypothetical protein | ||||
| PA3333 | fabH2 | 6.6 | 52 | 6.0 | 3-Oxoacyl-acyl carrier protein synthase III | |||
| PA3334 | 20 | Probable acyl carrier protein | ||||||
| PA3335 | 19 | Hypothetical protein | ||||||
| PA3347 | 3.1 | Hypothetical protein | ||||||
| PA3361 | lecB | 26 | 6.0 | Fucose-binding lectin PA-IIL | ||||
| PA3369 | 9.4 | 11 | 6.3 | Hypothetical protein | ||||
| PA3370 | 5.2 | 4.8 | Hypothetical protein | |||||
| PA3418 | ldh | 10 | 4.2 | 3.3 | Leucine dehydrogenase | |||
| PA3476 | rhlI | 11 | Autoinducer synthesis protein RhlI | |||||
| PA3477 | rhlR | 6.5 | 2.7 | 12 | Transcriptional regulator RhlR | |||
| PA3478 | rhlB | 12 | 9.3 | 57 | Rhamnosyltransferase chain B | |||
| PA3479 | rhlA | 28 | 16 | 46 | Rhamnosyltransferase chain A | |||
| PA3520 | 38 | 9.0 | Hypothetical protein | |||||
| PA3535 | 10 | 12 | Probable serine protease | |||||
| PA3688 | 4.0 | Hypothetical protein | ||||||
| PA3691 | 11 | 8.7 | 2.9 | 8.5 | Hypothetical protein | |||
| PA3692 | 8.2 | 6.5 | 7.8 | Probable outer membrane protein precursor | ||||
| PA3724 | lasB | 413 | 4.0 | 597 | Elastase LasB | |||
| PA3888 | 3.4 | 2.8 | Probable permease of ABC transporter | |||||
| PA3904 | 9.9 | 21 | Hypothetical protein | |||||
| PA3906 | 9.5 | 21 | Hypothetical protein | |||||
| PA3907 | 7.4 | 24 | Hypothetical protein | |||||
| PA3923 | 5.9 | 27 | Hypothetical protein | |||||
| PA4117 | 3.2 | Probable bacteriophytochrome | ||||||
| PA4129 | 6.7 | 4.1 | Hypothetical protein | |||||
| PA4130 | 7.2 | 3.8 | Probable sulfite or nitrite reductase | |||||
| PA4131 | 6.6 | 7.0 | Probable iron-sulfur protein | |||||
| PA4133 | 3.9 | 28 | 16 | Cytochrome c oxidase subunit (cbb3 type) | ||||
| PA4134 | 4.9 | 42 | Hypothetical protein | |||||
| PA4139 | 6.9 | 3.8 | Hypothetical protein | |||||
| PA4141 | 66 | 33 | 102 | Hypothetical protein | ||||
| PA4142 | 9.6 | 5.5 | Probable secretion protein | |||||
| PA4171 | 10 | 13 | Probable protease | |||||
| PA4175 | prpL | 19 | 52 | Pvds-regulated endoprotease, lysyl class | ||||
| PA4205 | mexG | 45 | 8.7 | 5.9 | Hypothetical protein | |||
| PA4206 | mexH | 11 | 6.7 | 9.2 | Probable RND efflux membrane fusion protein precursor | |||
| PA4207 | mexI | 15 | 8.4 | 6.2 | 4.9 | Probable RND efflux transporter | ||
| PA4208 | opmD | 4.3 | 8.0 | 5.5 | Probable outer membrane protein precursor | |||
| PA4209 | phzM | 31 | 37 | Probable phenazine-specific methyltransferase | ||||
| PA4210 | phzA1 | 19 | 27 | Probable phenazine biosynthesis protein | ||||
| PA4211 | phzB1 | 124 | 525 | Probable phenazine biosynthesis protein | ||||
| PA4217 | phzS | 55 | 69 | Flavin-containing mono-oxygenase | ||||
| PA4296 | 4.1 | 8.1 | Probable two-component response regulator | |||||
| PA4306 | 9.0 | Hypothetical protein | ||||||
| PA4311 | 13 | 4.0 | 2.9 | Conserved hypothetical protein | ||||
| PA4496 | 3.4 | Probable binding protein component of ABC transporter | ||||||
| PA4498 | 4.6 | Probable metallopeptidase | ||||||
| PA4590 | pra | 27 | 10 | Protein activator | ||||
| PA4648 | 10 | 18 | Hypothetical protein | |||||
| PA4649 | 19 | Hypothetical protein | ||||||
| PA4738 | 7.8 | 19 | 5.0 | 18 | Conserved hypothetical protein | |||
| PA4739 | 13 | 26 | 8.1 | 18 | Conserved hypothetical protein | |||
| PA4778 | 6.4 | 6.6 | Probable transcriptional regulator | |||||
| PA4869 | 5.4 | Hypothetical protein | ||||||
| PA4876 | osmE | 8.3 | 4.6 | Osmotically inducible lipoprotein OsmE | ||||
| PA4880 | 6.1 | 8.2 | Probable bacterioferritin | |||||
| PA5058 | phaC2 | 3.1 | 4.1 | Poly(3-hydroxyalkanoic acid) synthase 2 | ||||
| PA5061 | 3.1 | 2.9 | Conserved hypothetical protein | |||||
| PA5220 | 26 | 21 | Hypothetical protein | |||||
| PA5481 | 38 | 8.7 | 29 | Hypothetical protein | ||||
| PA5482 | 19 | 12 | 29 | Hypothetical protein | ||||
| QS repressed | ||||||||
| PA0887 | acsA | 3.5 | Acetyl-coenzymeA synthetase | |||||
| PA1559 | 15 | 4.1 | Hypothetical protein | |||||
| PA2007 | maiA | 15 | 16 | Maleylacetoacetate isomerase | ||||
| PA2008 | fahA | 8.1 | 6.9 | Fumarylacetoacetase | ||||
| PA2009 | hmgA | 7.8 | 9.5 | Homogentisate 1,2-dioxygenase | ||||
| PA2540 | 28 | Conserved hypothetical protein | ||||||
| PA3038 | 2.3 | 2.8 | Probable porin | |||||
| PA3234 | 3.6 | 4.0 | Probable sodium:solute symporter | |||||
| PA3235 | 2.9 | 2.9 | 5.4 | Conserved hypothetical protein | ||||
| PA3205 | 6.7 | 2.7 | Hypothetical protein |
a
These genes have been reported previously as being regulated by quorum sensing (either QS regulated or QS repressed) (43, 51). CoA, coenzyme A; MFS, major facilitator superfamily; FAD, flavin adenine dinucleotide.