TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| E. coli | ||
| IMW205 | dcuR::Km | 67 |
| VJS632 | F− λ− prototroph | 56 |
| VJS676 | As VJS632 but Δ(argF-lacIZYA)U169 | 56 |
| Derivatives of strain VJS676 | ||
| VJS2197 | λΦ(narG-lacZ) | 46 |
| VJS4033 | λΦ(narG-lacZ) ΔnarX242 narL505 ychO2084::Ω-Cm narQ251::Tn10d(Tc) | 46 |
| VJS4339 | λΦ(fdnG-lacZ) Δ(narXL-ychO)240::Km narQ251::Tn10d(Tc) narP253::Tn10d(Cm) | 47 |
| VJS4341 | λΦ(fdnG-lacZ) narQ251::Tn10d(Tc) narP253::Tn10d(Cm) | 47 |
| VJS3046 | λΦ(frdA-lacZ) | 47 |
| VJS3052 | λΦ(frdA-lacZ) narL215::Tn10 | 47 |
| VJS4330 | λΦ(frdA-lacZ) narP253::Tn10d(Cm) | 47 |
| VJS4331 | λΦ(frdA-lacZ) narL215::Tn10 narP253::Tn10d(Cm) | 47 |
| VJS8515 | λ− Δ(att-lom)::bla {Φ(aspA-lacZ)} | This study |
| VJS8535 | λΦ(frdA-lacZ) dcuR::Km | This study |
| VJS8537 | λ− Δ(att-lom)::bla {Φ(aspA-lacZ)}/dcuR::Km | This study |
| VJS8559 | λ− Δ(att-lom)::bla {Φ(aspA-lacZ)}/narL215::Tn10 | This study |
| VJS8561 | λ− Δ(att-lom)::bla {Φ(aspA-lacZ)}/narL215::Tn10 narP253::Tn10d(Cm) | This study |
| VJS8566 | λ− Δ(att-lom)::bla {Φ(aspA-lacZ)}/narP253::Tn10d(Cm) | This study |
| VJS9009 | λ− Δ(att-lom)::bla {Φ(dcuS-lacZ)} | This study |
| VJS9010 | λ− Δ(att-lom)::bla {Φ(dcuS-lacZ)}/narL215::Tn10 | This study |
| VJS9011 | λ− Δ(att-lom)::bla {Φ(dcuS-lacZ)}/narP253::Tn10d(Cm) | This study |
| VJS9012 | λ− Δ(att-lom)::bla {Φ(dcuS-lacZ)}/dcuR::Km | This study |
| VJS9016 | λ− Δ(att-lom)::bla {Φ(dcuS-lacZ)}/narL215::Tn10 narP253::Tn10d(Cm) | This study |
| Plasmids | ||
| pRS415 | Apr; lacZ operon fusion vector | 49 |
| pVJS3266 | Apr; Δ(lacY lacA cynX tet) derivative of pRS415 | This study |
| pVJS4042 | As pVJS3266 but Φ(aspA-lacZ) | This study |
| pVJS4043 | As pVJS3266 but Φ(dcuS-lacZ) | This study |