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The common structures of the delivery systems. (A) Lipid nanoparticles (LNPs) are typically prepared by microfluidics with traditional (cationic or ionizable) lipids or functional lipids, cholesterol, and helper lipids. (B) The polymer‐based vectors, including polyethyleneimine (PEI), 74 ionizable amphiphilic Janus dendrimer (IAJD), 81 and chitosan, can encapsulate mRNA to form stable nanoparticles via simple mixing.
