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. 2024 Dec 20;11:1511682. doi: 10.3389/fnut.2024.1511682

Figure 7.

Figure 7

Sesamin activates autophagy by regulating TFEB nuclear translocation. (A) Protein levels of TFEB and LAMP1; (B) Semiquantitative analyses based on A; (C) Protein levels of TFEB in nuclear and cytoplasm; (D) Relative protein level of TFEB in nuclear; (E) LAMP1 immunofluorescence; (F) Semiquantitation on LAMP1 immunofluorescence; Scale bar = 50 μm. 9-trans-C18:1: 500 μM 9-trans-C18:1 + RPMI 1640; S32: 500 μM 9-trans-C18:1 + 32 μM sesamin; EO: 10 μM EO+ 500 μM 9-trans-C18:1 + 32 μM sesamin; MHY1485: 2 μM MHY1485+ 500 μM 9-trans-C18:1 + 32 μM sesamin. All data are represented as means ± SD (n = 3–5), *p < 0.05, **p < 0.01.