TABLE 1.
Primers used for the RT-PCR on the map1 cluster of E. ruminantium Senegal and Gardel
| Genea | Senegal (5′-3′) | Gardel (5′-3′) |
|---|---|---|
| map1-14 (f) | TGTTGACTTTTCCAATGAGAGTGA | TGTTGACTTTTCCAATGAGAGCGA |
| (r) | TTGGTGAAAGTAAAAACCCCATAC | TTGGTGAAAGTAAAAACCCTACAC |
| map1-13 (f) | AGATCTTAAAGAGGATGGGTACAA | AGATCTTAAAGAGGATGGATACAA |
| (r) | GGTAATAACCTTCTGCAACAGCTA | CTAAAATAAACCTTACTCCTATAC |
| un-1 (f) | ATTAACAGCACTCCCAATCCATTA | ATTAGCAGCACTCCTAATCCAGTA |
| (r) | AATTTGTACAGCTGCTTGGAAAAA | GGAAAACAACACTTTTTGTGGGTG |
| map1-12 (f) | AATACAAGCCAAGCATTTCGTACT | AATACAAGCCAAGCATTTCGTATT |
| (r) | TAACATTGAATTTTGCTGATGATG | TAACGTTGAATTTTGCTGATGATG |
| map1-11 (f) | TATCAAACTTTCGAAACTTCCACA | TATCAAACTTTCGAAACTTTCACA |
| (r) | CACTGATCAGGAGATTTGTTCTTG | CACTGATCAAGAGATTTGTTCTTG |
| map1-10 (f) | ATTACCACCCAATTTAAGCCTACT | ATTACCAGCCAATTTAAGCCTACT |
| (r) | AATCCTTAACCCGACTTCGCTACC | AATCCTTAACCCGACTTCGCTACC |
| map1-9 (f) | GTAACGTACCAAATACTGAAGATG | GTAACGTACCAAATACTGAAGATG |
| (r) | TATTAAGTTTGGCTATTGCTGAA | TATTAAGTTTGGCTATTGCTGAAG |
| map1-8 (f) | TTTAACATTCTTATTAGCGTTTTC | TTTAACATTCTTATTAGCGTTCTC |
| (r) | CTGATTTTAGGAGATACGCGATAA | CTGATTTTAGGAGATACACGATAA |
| map1-7 (f) | TGGACAATATAAGCCAGGAGTTCC | TGGACAATATAAGCCAGGAGTTCC |
| (r) | ACTGCACTAGTAATTTTGGGTTCA | GTTGCACTAGTAATTTTGGGTTCA |
| map1-6 (f) | TTTTTACCTCACCAAGCACTTTC | TTTTTACCTCACCAAGTACTTTC |
| (r) | AGCTAATGTTTAACGTTGATGTTG | AGCTAATGTTTAATGTTGATGTTG |
| map1-5 (f) | CTTTTGCCTTCATTCATACATTTA | ATACAAAACTAGAAAAAGAGAATA |
| (r) | TACTTACTTTGACATTATATGCTA | TACTTACTTTGACATTATATGCTA |
| map1-4 (f) | GTTTCAGTGGCACTATAGGACAGT | GTTTTAGTGGCACTATTGGACAGT |
| (r) | TTGGGATTATTTGCAAGCATACGA | TTGGGATTATTTGCAAGCATACGA |
| map1-3 (f) | CAATTTTCGTAACTTCTCAGTCAA | CAATTTTCGTAATTTCTCAGTTAA |
| (r) | TTTAAATTTATTGCCTGCTACTTT | TTTAAATTTATTACCTACTACTTT |
| map1-2 (f) | AATAAACTCATTGCAACAGGTATA | AACAAACTCATTGCAACAGGTATA |
| (r) | CTAATGGGATGTTATAGAGATCAG | CTAATGGGGTGTTATTCAGATCAG |
| map1-1 (f) | CCAAGCATACCACATTTCAGA | CCAAGCATACCACACTTCAGA |
| (r) | TGAAGCGGAAGTGCTTTGAGG | TGAAGCGGAAGTGCTTTGAGG |
| map1 (f) | TAATATCATTAGTGTCATTTTTACC | TAATATCATTAGTGTCATTTTTACC |
| (r) | TGGACTAACAGCACTACTGGC | GTGTTGCTGATGCAAAACCTGG |
| map1+1 (f) | CTTAGTTAAACCAGGGATTG | ATAATCAATATCTAAATTAGCAGA |
| (r) | AGGTGTTACATTAAGTGCAT | GGTGTTACATTAAGTGCATTGTTG |
a
f, forward; r, reverse.