TABLE 3.

Transcription of the E. ruminantium map1 multigene family in infected tick cell lines

Gene	Cell line, cell line passage no., isolate, and isolate passage no.a
	AVL13		IDE8		IRE18	RAN3			RAE1	RAE25	BME2	BDE16
	18	18	82	83	23	56	61	56	85	147	78	26
	G	W	G	W	G	G	W	W	W	W	G	G
	0 (5)b	0 (2)	5	6	5	14 (7)	2 (1)	5 (1)	1 (1)	1 (1)	5 (4)	8 (4)
map1-14	+	+		+							+	+
map1-13	+	+	+	+			+	+		+		+
un-1	+	+		+			+	+
map1-12											+
map1-11
map1-10					+						+	+
map1-9	+	+	+	+		+	+	+				+
map1-8	+	+	+	+		+	+	+
map1-7	+	+	+	+		+	+	+	+		+	+
map1-6				+	+		+				+	+
map1-5											+	+
map1-4	+	+	+	+			+		+		+	+
map1-3
map1-2		+		+			+	+	+
map1-1	+	+	+	+	+	+	+	+	+	+	+	+
map1	+	+	+	+			+	+	+	+		+
map1+1	+	+	+	+	+	+	+	+	+	+	+	+

^aInfected cell lines IDE8, RAN3, RAE1, RAE25, BME2, and BDE16 were incubated at 31°C, IRE18 at 28°C, and AVL13 at 37°C. “/CTVM” has been omitted from cell line names for simplicity. G, CTVM Gardel lacking map1-2; W, Welgevonden.

^bThe first number indicates the E. ruminantium passage level in the cell line, the number in parentheses is the passage level in the IDE8 culture used to infect the cell line. IDE8 and IRE18 were infected directly from bovine endothelial cells (4).