Skip to main content
. 2025 Jan 2;26(1):68–81. doi: 10.1038/s41590-024-02034-1

Extended Data Fig. 4. Basic characterization of in vivo model and additional in vivo effector/memory phenotyping at high CAR dose.

Extended Data Fig. 4

Data are related to Fig. 2. 4A: Basic flow cytometry gating strategy for in vivo experiments. Total events were gated by Singlets, Live Cells and then Lymphocytes, followed by CD8a+/TCRbeta+/EGFR+ cells for CAR8/EGFR8 or B220+/CD22+ cells for E2A-PBX. S4C-F are from experiments with the 1e6 EGFR+ cell dose. 4B: Proportions of CAR8 with the short-lived effector cell (SLEC, IL7Ra-/KLRG1+) or memory precursor effector cell (MPEC, IL7Ra+/KLRG1-) phenotypes at the indicated timepoint against WT leukemia. (Exact p values, left to right then top to bottom: <0.0001, <0.0001, <0.0001, <0.0001, <0.0001, 0.0005, 0.0003, 0.0016, <0.0001, <0.0001). 4C: Proportions of CAR8 with the short-lived effector cell (SLEC, IL7Ra-/KLRG1+) or memory precursor effector cell (MPEC, IL7Ra+/KLRG1-) phenotypes at the indicated timepoint against CD19Lo leukemia. (Exact p values, left to right then top to bottom: 0.0001, <0.0001, <0.0001, <0.0001, <0.0001, 0.0004, 0.0002, 0.0363, <0.0001, 0.0001). 4D: Proportions of CAR8 with the effector memory precursor (EMP, CD27+/CD62L-) or central memory precursor (CMP, CD27+/CD62L+) phenotypes at the indicated timepoint against WT leukemia. (Exact p values: All <0.0001). 4E: Proportions of CAR8 with the effector memory precursor (EMP, CD27+/CD62L-) or central memory precursor (CMP, CD27+/CD62L+) phenotypes at the indicated timepoint against CD19Lo leukemia. (Exact p values, left to right then top to bottom: <0.0001, <0.0001, 0.0001, <0.0001, <0.0001, 0.0003, <0.0001, <0.0001, <0.0001, <0.0001). Data in S4B-E are from 2 pooled, independent experiments with n = 10 mice per condition. Statistical comparisons were performed using one-way Brown-Forsythe and Welch ANOVA with two-sided Dunnett’s T3 multiple comparisons test. Data represent mean +/− SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Source data