Table 2.
PCR primers for real-time PCR assay.
| Target species | Primer sequence (5′) | GeneBank accession no. | TE (°C) | Size (bp) |
|---|---|---|---|---|
| Total bacteria | F: CGGCAACGAGCGCAACCC R: CCATTGTAGCACGTGTGTAGCC |
AY548787.1 | 60 | 147 |
| Total anaerobic fungi | F: GAGGAAGTAAAAGTCGTAACAAGGTTTC R: CAAATTCACAAAGGGTAGGATGATT |
GQ355327.1 | 57.5 | 120 |
| Total protozoa | F: GCTTTCGWTGGTAGTGTATT R: CTTGCCCTCYAATCGTWCT |
HM212038.1 | 59 | 234 |
| Total methanogens | F: TTCGGTGGATCDCARAGRGC R: GBARGTCGWAWCCGTAGAATCC |
GQ339873.1 | 60 | 160 |
| R. albus | F: CCCTAAAAGCAGTCTTAGTTCG R: CCTCCTTGCGGTTAGAACA |
CP002403.1 | 60 | 176 |
| R. flavefaciens | F: ATTGTCCCAGTTCAGATTGC R: GGCGTCCTCATTGCTGTTAG |
AB849343.1 | 60 | 173 |
| B. fibrisolvens | F: ACCGCATAAGCGCACGGA R: CGGGTCCATCTTGTACCGATAAAT |
HQ404372.1 | 61 | 65 |
| F.succinogenes | F: GTTCGGAATTACTGGGCGTAAA R: CGCCTGCCCCTGAACTATC |
AB275512.1 | 61 | 121 |
| R. amylophilus | F: CTGGGGAGCTGCCTGAATG R: GCATCTGAATGCGACTGGTTG |
MH708240.1 | 60 | 102 |
| P. ruminicola | F: GAAAGTCGGATTAATGCTCTATGTTG R: CATCCTATAGCGGTAAACCTTTGG |
LT975683.1 | 58.5 | 74 |