TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| XL10-Gold | Stratagene | |
| BL21(DE3) codonPlus-RIL | Stratagene | |
| B. subtilis | ||
| JH642 | trpC2 pheA1 | Lab stock |
| JH19000 | JH642asigB::cat | 11 |
| LM58 (ΔyfkJ) | JH642::pLM58 yfkJ::[erm PspaclacZ] | This study |
| LM24 (ΔywlE) | JH642::pLM24aywlE::spc | This study |
| LM02 | JH642::pLM02ayfkJ::cat | This study |
| LM50 | LM02::pLM24ayfkJ::cat ywlE::spc | This study |
| LM08 | JH642::pLM06B amyE::[yfkJp-lacZ::aph] | This study |
| LM14 | JH19000::pLM06B sigB amyE::[yfkJp-lacZ::aph] | This study |
| LM35 | JH642::pLM35 amyE::[ywlEp-lacZ::aph] | This study |
| LM60 | JH19000::pLM35 sigB amyE::[ywlEp-lacZ::aph] | This study |
| Plasmids | ||
| pEGST | GST gene in pET23b (Novagen) | 26 |
| pET15b | Cleavable N terminus His6 tag | Novagen |
| pJM134A | Spectinomycin cassette in pBluescriptIIKS (+) | Unpublished |
| pMUTIN2 | PspaclacZ + erythromycin cassette | 32 |
| pJM115 | lacZ transcriptional fusion that integrates in amyE | 40 |
| pLM58 | Construct for nonpolar deletion of yfkJ in pMUTIN2 | This study |
| pLM24 | Left and right arms of ywlE in pJM134 | This study |
| pLM02 | Left and right arms of yfkJ in pJM105A | This study |
| pLM06B | yfkJ promoter in pJM115 | This study |
| pLM35 | ywlE promoter in pJM115 | This study |
a
This strain was transformed with linearized plasmids.