TABLE 2.
Phenotypic characterization of strain PAO1 and derivatives
| Strain (genotype) | Concn of:
|
Elastase activityc (A495) | Amt of rhamnolipidsd (μg/109 bacteria) | HCN concne (nmol/109 bacteria) | Growth on adenosinef | ||
|---|---|---|---|---|---|---|---|
| Pyocyanina (μg/ml) | OdDHLb (μM) | BHLb (μM) | |||||
| PAO1 | 4.7 ± 0.6 | 0.22 ± 0.06 | 3.9 ± 0.2 | 0.9 ± 0.2 | 7.5 ± 2.2 | 2.90 ± 0.30 | +++ |
| PAO1-J (lasR) | 20.7 ± 1.1 | <0.05 | 0.2 ± 0.1 | 0.1 ± 0.1 | <1.0 | 0.63 ± 0.15 | + |
| PAO1-D (lasR) | 20.6 ± 2.8 | <0.05 | <0.1 | 0.1 ± 0.0 | <1.0 | 0.43 ± 0.06 | + |
| PAO6395 (ΔlasR) | 15.9 ± 3.4 | <0.05 | 0.1 ± 0.1 | 0.1 ± 0.0 | <1.0 | 0.40 ± 0.00 | + |
Pyocyanin was quantified in triplicate cultures grown for 48 h as previously described (46).
OdDHL and BHL concentrations were quantified by thin-layer chromatography, using Agrobacterium tumefaciens NTL4/pZLR4 and Chromobacterium violaceum CV026 as indicators, as previously described (18). Assays were done three times.
Elastase activity was estimated for P. aeruginosa strains grown in triplicate NYB cultures (7, 33). Arbitrary A495 units are used.
Rhamnolipids were quantified (19) for P. aeruginosa strains grown in triplicate NYB cultures.
Concentrations of HCN were determined in triplicate for strains grown under semianaerobic conditions (19).
Growth on OS medium with adenosine as the only C source was scored after 2 days. +++, good growth of single colonies; +, faint growth in primary streak.