FIGURE 2.

AEA reduces chemokine production in Zymosan-activated Macrophages. Human primary macrophages were treated with ethanol (EtOH), Diclofenac and EtOH (Dic), or and AEA (10 µM) with Diclofenac (AEA), and were subsequently stimulated with Zymosan for 6 h. The concentrations of CCL2 (A), CCL4 (B), CCL20 (C), and CCL17 (D) were determined by LegendPlex assay. Besides raw data, normalized data of Zymosan-activated cells is shown. Data are from three independent experiments, with at least 2 donors each. Each data point corresponds to a single donor (n = 7). Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; p-values were calculated using two-way ANOVA or one-sample t-test.