FIGURE 6.

AEA has an impact on the proliferation, activation and exhaustion profiles of CD8 T cells. Human isolated T cells were pre-treated with ethanol (EtOH), Diclofenac and EtOH (Dic), and AEA (10 µM) with Diclofenac (AEA), and were activated or remained inactivated for up to 6 days. Cells were measured by flow cytometry. Percentage of CD8⁺ T cells (A), PD1+ CD8⁺ T cells (B), TIGIT+ CD8⁺ T cells (C), Tc17 cells (D), Tc1 cells (E), CD38⁺ CD8⁺ T cells (F), and CD69⁺CD8⁺ T cells (G) are shown. (H) Representative FACS plot of CD69⁺CD8⁺ T and CD38⁺CD8⁺ T cells. Data are from three independent experiments, with at least 3 donors each. Each data point corresponds to a single donor (n = 11). Data are shown as mean $\pm$ SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; p-values were calculated using two-way ANOVA with Tukey correction.