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. 2025 Jan 2;15:351. doi: 10.1038/s41598-024-82748-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — DNA cloning, the purification of proteins, and the determination of pI. (A) Description of the upstream and downstream sequences of blaGOB-38 and the experimental steps for overexpression of the GOB-38 protein. (B) SDS-PAGE after the different purification steps. M, molecular mass standards; lane 1, crude extract from BL21-DE3; lane 2, Ni-NTA agarose resin eluent; lane 3, STarm Streptactin beads flow through liquid; lane 4, STarm Streptactin beads detergent; lane 5–7, STarm Streptactin beads eluent. (C) GOB-38 pI determination. Red arrow, standard; black arrow, sample.