Table 5.
Bacterial strains and plasmids used in this study.
| Strains and plasmids | Description | Source |
|---|---|---|
| EA-1 | The wild-type strain of E. anophelis | Isolated from patient sputum |
| Ab6-2 | The wild-type strain of A.baumannii | Isolated from patient sputum |
| DH5α | E. coli DH5α as a host cell for cloning of the blaGOB-38 gene | Our laboratory collection |
| BL21-DE3 | E. coli BL21-DE3 as a host cell for expression of GOB-38 | Our laboratory collection |
| ATCC 25,922 | E. coli ATCC 25,922 as quality control for antimicrobial susceptibility testing | Our laboratory collection |
| Ab5075 | A.baumannii 5075 as nitrocefin chromogenic test positive control | Our laboratory collection |
| ATCC17978 | A. baumannii as a host cell for cloning of the blaGOB-38 gen | As a gift from Professor Yu Yunsong |
| pK-18mob | Cloning vector for the PCR product of the blaGOB-38 gene with its upstream promoter region, Kanr | Our laboratory collection |
| pET28a | Expression vector for the PCR product of the ORF of the blaGOB-38 gene, Kanr | As a gift from Professor Zheng Fanglin |
| pK-blaGOB-38(G) | The recombinant plasmid harbors the blaGOB ORF and putative promoter region | Constructed in this research |
| pK-blaGOB-38(A) | The recombinant plasmid harbors the blaGOB ORF and putative promoter region, RBS was mutated | Constructed in this research |
| pET28a-blaGOB-38 | The recombinant plasmid harbors the blaGOB ORF | Constructed in this research |
r, resistance; Kan, Kanamycin; ORF, open reading frame; RBS, ribosome binding site.