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. 2025 Jan 2;16:276. doi: 10.1038/s41467-024-55661-5

Fig. 4. GSSG but not GSH administration improves cardiac function after MI through Drp1 S-glutathionylation.

Fig. 4

A, B Representative images of supersulfide A and hydrogen sulfide B in mouse heart slice 5 weeks after MI. (n = 3 mice per treatment). Supersulfides and hydrogen sulfide were detected using SSip-1 and SF7-AM probes, respectively. The yellow dashed line shows the border of the scar and non-scar area. Scale bars, 1 mm. C Drp1 S-glutathionylation in mouse heart at 3 days after GSSG administration. Bands corresponding to Drp1 S-glutathionylation (arrowhead) were quantified (n = 5 mice per treatment). D, E Changes in fractional shortening (FS) D and ejection fraction (EF) E in mice after MI. An osmotic pump filled with saline, GSSG or GSH (30 mg/kg/day) was implanted intraperitoneally at 7 days after MI. (n = 6 mice for MI + GSH, n = 5 for others). F Effect of GSSG or GSH on heart weight (HW) / tibia length (TL) ratio in mice 5 weeks after MI. (n = 6 mice for MI + GSH, n = 5 for others). G Representative electron micrographs of peri-infarct zone myocardium. The average size of mitochondrial area was quantified. (n = 5 mice per treatment). Scale bars, 2 µm. H–K Quantification of complex I activity of mitochondrial supercomplex (H), mtDNA/nDNA ratio (I), NADH/NAD+ ratio J, 4-HNE intensity K in myocardium. (n = 5 mice per treatment). L Effect of GSSG or GSH on the proportion of SA-β-gal-positive area in peri-infarct zone myocardium 5 weeks after MI. (n = 6 mice for MI + GSH, n = 5 for others). Scale bars, 200 µm. M Plasma BUN levels measured 5 weeks after MI. (n = 5 mice per treatment). Data are shown as the means ± SEM. Significance was determined by two-sided unpaired t-test A–C; one-way ANOVA followed by Tukey’s post-hoc test F–M; two-way ANOVA followed by Sidak’s post-hoc test D, E. Source data are provided as a Source Data file.