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Melting temperature as determined by CD and equilibrium dissociation constants as determined by SPR.
| Affibody | Tm (°C) | KD ± SD (nM) |
|---|---|---|
| ZDGCR2:3D1 | 69.0 | 26.7 ± 3.8 |
| ZDGCR2:AM106 | 56.4 | 2.4 ± 0.3 |
| ZDGCR2:AM115 | 64.0 | 2.1 ± 0.1 |
| ZDGCR2:AM156 | 60.0 | 8.5 ± 0.1 |
