Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2025 Jan 2;16:142. doi: 10.1038/s41467-024-55597-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Heatmap of regulon activity that co-occurs with known TF markers for LP and JZP identities. JZP identity was assigned by detecting clusters with high activity of the Ascl2 regulon (c3), and LP identity by the Gcm1 regulon (c4). Difference between JZP cluster activity and LP cluster activity was calculated for each regulon. Top 10 regulons for highest and lowest ratio, as well as top 10 regulons with highest activity in t0 cluster (cluster 9) were selected for the heatmap. Colour represents average score for the activity of a regulon for each cluster. All values were mean centred for each regulon and number of genes in the regulon is shown in brackets. The suffix _ext. in the regulon name indicates lower confidence annotations (by default inferred by motif similarity) are also used. b-d Specific SCENIC transcription factor regulons co-localize with TSCs, and cells associated with JZP and LP lineage. Cells were coloured by AUC score for regulons of interest. (b) UMAP plot of Inhibit dataset coloured by regulon activity. The TSCs-specific regulons Eomes are coloured blue, JZP regulon Ascl2 is coloured green, and LP regulon Gcm1 is in pink. c UMAP plot of Inhibit dataset coloured by E2f8 regulon activity. E2f8 regulon activity overlaps Gcm1 activity. d UMAP plot of Inhibit dataset coloured by Phf8 regulon activity. Phf8 regulon activity is increased over time in LP cells and overlaps with E2f8 and Gcm1 activity.