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. 2025 Jan 2;16:167. doi: 10.1038/s41467-024-55408-2

Fig. 3. VX-147 inhibits APOL1-mediated flux and cell death.

Fig. 3

A The graph shows baseline normalized APOL1-mediated ionic current measured for HEK293 cell lines expressing APOL1 G0, APOL1 G1, and APOL1 G2 variants, as a function of compound concentration. Whole cell currents were measured for these experiments. The first concentration point is no VX-147 and was artificially assigned as 2 orders of magnitude smaller than the lowest concentration tested for graphical representation. Multiple cells were recorded for each APOL1 variant, n = 8 for APOL1 G0, n = 11 for APOL1 G1 and n = 14 for APOL1 G2. B Assessment of Trypanosoma brucei brucei cell viability in the presence of recombinant APOL1 of the indicated variant using an alamarBlue™ assay. Experiments were performed in duplicate. Experimental replicates performed were n = 1 for APOL1 G0, n = 4 for APOL1 G1 and n = 1 for APOL1 G2. C Assessment of cell viability for HEK293 cell lines expressing APOL1 G0, APOL1 G1, and APOL1 G2 variants using the Promega MultiTox fluorescence assay. Experiments were performed in duplicate. Experimental replicates performed were n = 1 for APOL1 G0, n = 4 for APOL1 G1 and n = 1 for APOL1 G2. Representative traces are shown for all experiments. Results are presented as mean ± SEM when more than 3 replicates or as mean only for replicates less than 3. Source data are provided as a Source Data file.