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. 2025 Jan 2;15:104. doi: 10.1038/s41598-024-84540-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — Divergence time estimation and phylogenetic relationships of PJ with related species. (A) Estimated divergence times were indicated near the nodes. Unit for marked time is Mya. Structure variations occurred in PJ were marked on the branches. Red, orange, and blue arrows indicated 17 kbp IR expansion, 7 kbp IR expansion, and three tRNA inversion, respectively. Two S. divaricata were collected from different accessions in Genbank; S.divaricata-1: KU866529, S. divaricata-2: MZ089852. (B) Phylogenetic relationships between plastome and 45S nrDNA sequences. Genbank ID of 45S nrDNA sequences of Pj-1, Pj-2, S. divaricata, and D. carota were KX757776, KX757777, KX757775, and MF185182 respectively. Bootstrap support value and posterior probabilities (BP/PP) are shown above the branches.